Introducing Single Nucleotide variations into the Vitamin C Transporter SLC23A
Bibliographic record
Abstract
The solute carrier SLC23A1, encoded by the SLC23A1 gene, is the key membrane transporter mediating the reabsorption of ascorbic acid, the reduced form of vitamin C, in the renal proximal tubule. It had been shown that a single nucleotide polymorphism (SNP) in SLC23A1 could alter the proteins ability to transport ascorbic acid. Recently, through various human re-sequencing projects, many novel single nucleotide variations (SNV) have been discovered and their information has been deposited in genomic databases. However, their impact on the proteins functions remains to be determined. In a first step for functional studies, SNV need to be introduced into an existing SLC23A1 expression plasmids. The objective of this study was to introduce selected SNVs into the coding region, or Open Reading Frame (ORF), of the SLC23A1 expression plasmid pReceiver-M55-SLC23A1. Six SNVs located proximal to the 5’ and 3’ ends of the SLC23A1 ORF were successfully introduced.
Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.
How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.001 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.001 |
| Insufficient payload (model declined to judge) | 0.001 | 0.001 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".