Structure function analysis of glutamate gated chloride channels
Bibliographic record
Abstract
AbsfracfGlutamate-gated c hloride c hannels ( GluCI) b elong t 0 the n icotinic l igand-gated ion channel family and are thus assumed to be heteropentamers.Each subunit contains a large extracellular N-terminal domain, four transmembrane do mains (TMI-TM4), and an extracellular C terminal.Caenorhabditis elegans expresses various GluCI channels formed by al, a2, a3, a4 and ~ subunits.The best understood GluCI channel is expressed in pharyngeal muscle cells where it mediates response to the M3 motor neuron.a2 forms this channel, probably in association with ~.The a2 mutant lacks M3 neurotransmission which can be rescued by pharynx-specifie a2 expression.My results show that al and a3 subunits cannot substitute for a2.Formation of chimeric constructs of al, a2 and a3 pinpoints the MI-M3 transmembrane region of a2 as the minimal rescuing domain.This region may therefore be important for localization or, in association with another subunit, in the formation of the active channel. II RésuméLes canaux perméables aux ions CC réglés par le glutamate (GluCI) font partie de la famille de canaux nicotiniques et sont donc possiblement des hétéropentamères.Chaque sous-unité contient un domaine extracellulaire volumineux à l'extrémité Nterminale de la protéine, quatre domaines transmembranaires (TMl-TM4), et un domaine extracellulaire à l'extrémité C-terminale.Le nématode Caenorhabditis elegans exprime divers canaux GluCI constitués des sous-unités al, a2, a3, a4 et ~.Le canal GluCI est exprimé dans des cellules musculaires du pharynx où il facilite la réponse au neurone moteur M3.Ce canal est un complexe de sous-unités a2, probablement en association avec des sous-unités ~.Un mutant de la sous-unité a2 ne répond pas à l'activité inhibitrice de M3.Ce mutant peut-être secouru p ar l'expression dans le pharynx de la sous-unité a2 de type sauvage.Mes résultats démontrent que les sous-unités al et a3 ne peuvent pas substituer le besoin fonctionnel de a2.L'analyse de constructions chimériques de al, a2 et a3 semble établir que la région transmembranaire Ml-M3 de la sous-unité a2 est le domaine minimal pour la fonction du canal.Cette région p ourraît donc être importante soit pour la formation de canaux actifs, en association avec d'autres sous-unités, soit pour la localisation des canaux le long de la membrane plasmique.III A cknowledgmen ts 1 want to thank everyone who played a role in getting my project done.A special thank you to my supervisor Joe Dent for his guidance and encouragement.Many thanks also to my lab people, in particular Mark Pellegrino, Aamna Kaul, Kate Steger and Beibei Zhao for their help and support. 1 would like to acknowledge the help ofpeople from other labs in particular Nikoleta Juretic from the Thomas Bureau lab for sequencing,
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.002 | 0.001 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".