Study of Canola Meal Extract in the Synthesis of Lipids by Mortierella alpina
Bibliographic record
Abstract
Quality and cost-effective raw materials and the utilization of agriculture by-products with potential\nquality attributes and negligible commercial value can be supplemented in several microbial\nprocessing industries. Canola meal (CM), a by-product of canola processing, has an excellent\nnutritional profile composed of protein, carbohydrates, vitamins, and minerals, and could serve as a\npotential candidate for microbial culture media. Saskatchewan, as a global leader in canola\nproduction, has plans for upscaling its canola processing facilities. The efficient utilization of canola\nmeal extract (CME) in the production of lipids and polyunsaturated fatty acids, such as arachidonic\nacid (ARA) has the potential to serve as valuable ingredients or base chemicals for food, feed, and\nnutraceutical industries. This current study focused on the incorporation of alkaline extract (AE) and\nsubcritical extract (SE) of CM in a culture medium of an oleaginous fungus, Mortierella alpina\nATCC 32223, by replacing conventional yeast extract (YE) at a level of 50 % and 100 %. Extraction\nfor AE was conducted at 75 °C with a pH of 10.5, while for SE, it was carried out at 160 °C without\npH control. Initial trials were conducted to examine the effect of aeration at a level of 0.5 to 2.0 vvm\non fungal growth and it was found that increasing aeration rate increased fungal growth rate from\n0.16 day-1 to 0.39 day-1. Also, the ARA percentage in the lipid increased from 10.39% to 21.86%. It\nbecame evident that ARA production was positively correlated with biomass accumulation and its\nlipid content. During the replacement of YE by CME, it was observed that the 100% substitution of\nYE by CME yielded growth results similar to that of the control, which employed standard media\ncontaining YE. Moreover, AE- and SE-supplemented media exhibited a higher ARA accumulation\nof 144.91 mg/L and 165.8 mg/L, respectively, in comparison to standard media (124.87 mg/L).\nInterestingly, when glucose was omitted from the culture, media supplemented with 100% AE\nperformed better as compared to standard media. However, the limitation of glucose had an adverse\neffect on both the growth and lipid content of the culture.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.001 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".