Towards the Characterization of Putative Degradation Targets of Klebsiella pneumoniae Lon Protease by Quantitative Proteomics and in vitro Degradation Assays
Bibliographic record
Abstract
Klebsiella pneumoniae is an opportunistic bacterial pathogen, possessing numerous virulence factors to adapt to harsh host environments. Lon is an ATP-dependent serine protease in K. pneumoniae and was recently discovered to have a potential role in iron homeostasis. To identify potential targets of Lon impacting iron-homeostasis, this study used quantitative proteomics to identify proteins with significantly increased or decreased abundance binding to Nickel-Nitriloacetic acid agarose in the presence or absence of hexahistidine-tagged Lon. Candidate target proteins were purified and tested against Lon in vitro for degradation. Our results identified further links between Lon and iron-related proteins, while also uncovering a strong connection between Lon and sulfur metabolism/amino acid synthesis proteins in the Cys family. Overall, this work begins to test the potential interactions between Lon and proteins involved in iron homeostasis and uncovers a potential new role for Lon in sulfur homeostasis.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.001 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.002 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.001 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".