A behavioural and morphological analysis of compromised primary afferent C-fibre input to the superficial dorsal horn of the rat spinal cord
Bibliographic record
Abstract
Neuropathic pain is caused by direct or indirect damage of the central nervous system or of primary afferent fibres (PAF). Small diameter C-fibres (C-PAF) are of great interest as they are responsible for the transduction of pain-related signals arising from the periphery. The adult C-fibre neuronal populations can be distinguished by their neuropeptide expression profile. The peptidergic population expresses neuropeptides such as substance P (sP) and calcitonin gene-related peptide (CGRP). The non-peptidergic C-fibres express the cell surface glycoproteins binding the plant lectin IB4 and lack neuropeptides. Non-peptidergic afferents are known to play a role in nociceptive transmission; however our understanding of the nature of this information and how it is processed by these afferents in the spinal cord is limited. In this thesis, we focused on examining the morphological, neurochemical and behavioural consequences of compromised C-PAF input to the superficial dorsal horn. Our approach was two-fold: a) to study the effects of a constriction injury of the sciatic nerve; b to study the effects of selectively ablating the IB4-binding sub-population of C-PAFs by means of an intra-sciatic injection of IB4-Saporin. In the peripheral nerve lesion study we examined the time-related effects of a constriction neuropathy on the peptidergic and non-peptidergic C-PAFs using neurochemical markers for each neuronal population. We observed a transient loss of IB4-binding in lamina II, resultant from the degeneration of CIa synaptic glomeruli. Paw withdrawal latencies to noxious heat stimuli were significantly reduced in neuropathic animals throughout the 3 week time course. Sensitivity to cold stimuli was also significantly altered in neuropathic animals. Paw withdrawal thresholds to innocuous and noxious mechanical stimulation were significantly reduced in neuropathic animals. We did not detect any change in the de
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.001 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.001 | 0.000 |
| Science and technology studies | 0.000 | 0.001 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.001 | 0.001 |
| Insufficient payload (model declined to judge) | 0.003 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".