Characterization of the lignification response in the wheat-Pyrenophora tritici-repentis system
Bibliographic record
Abstract
Lignin, a product of the phenylpropanoid pathway, is produced as part of a defence response in many plants. In the wheat-'Pyrenophora tritici-repentis ' system (a) the time and magnitude of indu tion of Phenylalanine Ammonia Lyase, Caffeic acid 'O'-Methyltransferase, Caffeoyl CoA 'O'-Methyltransferase, Cinnamyl Alcohol Dehydrogenase and Peroxidase enzymes and (b) the accumulation of 'PAL' and ' pox' genes transcripts were measured. The enzymes were assayed in the infected and control (water sprayed) leaf tissues of a resistant (Erik) and a susceptible (Glenlea) cultivar at 0, 24, 48 and 72 h post-inoculation. No difference in the time and magnitude of induction of PAL, COMT, CCOMT, CAD and POX enzymes were observed between Erik and Glenlea, when the activities were expressed on a tissue weight basis and on lesion area. 'PAL' northern analysis indicated the presence of higher levels of a 2.4 Kb ' PAL' gene transcript in the infected leaf tissue of Erik compared to Glenlea at 48 host-inoculation. 'pox' RT-PCR analysis indicated (a) no expression of 'pox1, pox4' genes; (b) a prominent 677 bp 'pox2' PCR product in the infected tissue of both resistant and susceptible cultivars and (c) a 734 bp ' pox3' product only in the infected leaf tissue of Salamouni pox northern analysis indicated a lack of difference in the induction of 1.2 Kb transcript in the infected tissue of Salamouni and Glenlea and a weaker induction of the same transcript in the infected tissue of Erik. These results indicate a flow of the phenylpropanoid and lignin synthesis pathway, leading towards the synthesis of lignin in the infected tissue of both Erik and Glenlea. Hence, in the wheat-'Pyrenophora tritici-repentis' system, lignin synthesised in response to infection may not play a dominant role in providing resistance.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".