The dynamics of cadherin-mediated cell-cell adhesion tissue separation in Xenopus Laevis
Bibliographic record
Abstract
The formation and maintenance of boundaries between embryonic regions are crucial to preserve the organization of the embryo during development. One important parameter that needs to be regulated at the interface between cells of two different tissues is cell-cell adhesion. In the present study, we attempted to understand and characterize the dynamics of cadherin-mediated cell-cell adhesion at the ectoderm-mesoderm boundary of the Xenopus gastrula. At this boundary, it has been recently shown that cells undergo cycles of attachments and detachments as the result of ephrin/Eph signaling (Rohani, Canty, Luu, Fagotto, & Winklbauer, 2011). Using three-dimensional live confocal microscopy, we found that the total levels of cadherin at the membrane do not change reproducibly during local repulsive events between ectodermal and mesodermal cells. Cadherin clusters, however, appear during contact establishment and often begin to fade away prior to the start of cell detachments. While the role of acto-myosin contraction in this process is still not fully elucidated, we found that cortical actin was upregulated during cell detachments, and that phospho-myosin was enriched at the endogenous ectoderm-mesoderm boundary. Furthermore, our data suggest that there may be two pools of activated myosin, and that the one that is most prominent at the boundary is the one involved in global cortical tension.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.001 | 0.000 |
| Meta-epidemiology (narrow) | 0.001 | 0.000 |
| Meta-epidemiology (broad) | 0.001 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.001 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.001 | 0.000 |
| Research integrity | 0.001 | 0.001 |
| Insufficient payload (model declined to judge) | 0.001 | 0.001 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; both teacher heads agree on what is shown here.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".