Effects of fibroblast growth factor 8 and 18 on ovine ovarian granulosa cell function
Bibliographic record
Abstract
Fibroblast Growth factors (FGFs) are growth factors which have diverse biological activities including broad mitogenic and cell survival activities. FGFs constitute a large family of 22 distinct polypeptide growth factors varying in size from 17 to 34 kDa and have between 13 to 71% sequence homology. More specifically, FGF8 and FGF18 are both important modulator of granulosa cell functions. FGF8 and FGF18 are homologous factors which possess similar sequence homology, but we hypothesized they may interact to FGFRs differently leading to distinct effects, particularly on granulosa cell growth and induce proliferation following a short period of exposition. This study was performed to investigate the effects of FGF8 and FGF18 on ovine granulosa cells proteome. Ovine ovaries were obtained from adult sheep’s irrespective of stage of estrous cycle and were cultured using a standard protocol. Granulosa cells were harvested from follicles then, seeded and cultivated. After, they were exposed to 10 ng/mL of FGF8 or FGF18. Cell proteins were extracted, cysteine bonds were reduced and acetylated and proteins were digested with trypsin. Tryptic peptides were analyzed using a bottom-up proteomic approach, mass spectrometry and a label-free quantitation method. The results obtained revealed following treatment with FGF8 or FGF18 for 30 minutes, an important shift toward upregulation for the entire granulosa cell proteome was measured. Additionally, several proteins, including ATF1, STAT3, MAPK1, MAPK3, MAPK14, PLCG1, PLCG2, PKCA, PIK3CA, RAF1, GAB1 and BAG2 were significantly upregulated (> 1.5-fold; p < 0.01). Results are suggesting the activation of the MAPK/ERK pathway as expected. However, it is important to note that ATF1 and STAT3 are important transcription factors involved in cell growth, proliferation and survival and consequently can hamper or rescue the normal ovine reproductive system function. Both are strongly interacting with the MAPK/ERK pathway. They are directly involved in the growth, proliferation and mechanisms of cell survival. This very significant increase of STAT3 and ATF1 could have important consequences on the viability of the oocyte.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.004 | 0.001 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".