Expression and purification of recombinant HIV-1 BH10 Tat protein
Bibliographic record
Abstract
The Human Immunodeficiency Virus (HIV) is the causative agent of acquired immune deficiency syndrome (AIDS). The HIV-1 BH10 genome encodes a two-exon gene for an 86 amino acid viral transactivator protein (Tat) that markedly enhances viral transcription. The objective of the present research was to develop an expression and purification system to produce HIV-1 BH10 Tat1-86 for structural analysis. We first attempted to express Tat protein with a PelB leader sequence for secretion into the periplasmic space of 'E. Coli'. We next expressed Tat with a cleavable 6-histidine purification tag at the amino terminus. The presence of several codons in the HIV-1 BH10 'tat' cDNA that are rarely used in ' E. coli' was investigated as a cause of low levels of protein expression. To this end, a 'tat' cDNA was produced by splicing nucleotides 1-186 of a Tat gene composed of frequently used 'E. coli' codons with nucleotides 187-258 of the HIV-1 BH10 'tat' cDNA. (Abstract shortened by UMI.)
Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.
How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.001 |
| Science and technology studies | 0.001 | 0.000 |
| Scholarly communication | 0.001 | 0.000 |
| Open science | 0.001 | 0.000 |
| Research integrity | 0.000 | 0.001 |
| Insufficient payload (model declined to judge) | 0.002 | 0.004 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".