Isolation and Characterization of the Corticotropin Releasing Factor-like Diuretic Hormone Receptor in Rhodnius prolixus
Bibliographic record
Abstract
Rhodnius prolixus is a hemipteran insect which undergoes rapid post-feeding diuresis following ingestion of a large blood meal that can be up to 10 times initial body weight. Corticotropin releasing factor-like diuretic hormone (Rhopr-CRF/DH) is involved in the neurohormonal synergism that increases rates of fluid secretion by Malpighian tubules over 1000 fold during rapid post-feeding diuresis. In this study, the Rhopr-CRF/DH receptor was isolated and characterized from fifth instar R .prolixus. The receptor is a family B G-protein coupled receptor (GPCR) and was deorphaned using a Chinese hamster ovary (CHO) cell line. RT-qPCR demonstrated strong receptor expression in the digestive tissues, upper Malpighian tubules and the reproductive tissues. Finally, a modified Human embryonic kidney (HEK293) cell line was used to verify the initiation of the cyclic adenosine monophosphate (cAMP) pathway upon activation of the receptor. Characterization of the CRF/DH receptor will serve to elicit better understanding into the precise coordination of diuresis and other physiological processes.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.001 |
| Insufficient payload (model declined to judge) | 0.001 | 0.001 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".