The role of the trehalose biosynthesis pathway in «Aspergillus fumigatus» development and stress response
Bibliographic record
Abstract
Trehalose is a non-reducing dissacharide of glucose involved in the metabolism of bacteria, plants, insects and fungi that is not present in mammals. In fungi, trehalose serves as source of energy for reproduction and development, and it can protect cells from environmental stress and nutrient limitations. It has also been linked with virulence in pathogenic yeasts. Trehalose biosynthesis in fungi involves a protein complex consisting of trehalose-6-phosphate synthase (Tps), trehalose-6-phosphate phosphatase (Tpp), and regulatory proteins. In the pathogenic mold Aspergillus fumigatus, we located four putative trehalose-6-phosphate synthases: tpsA, tpsB, tpsC, and tpsD. We found that trehalose accumulated during the developmental life cycle of A. fumigatus, throughout which both tpsA and tpsB were significantly expressed. In stress, trehalose accumulated only during heat shock, and this accumulation correlated with an increase in expression of tpsA. We therefore created strains of A. fumigatus in which tpsA, tpsB, or both were disrupted. Only the ΔtpsAB double mutant failed to accumulate trehalose under any conditions. The ΔtpsAB mutant was also delayed in germination at normal growth temperature, suggesting a developmental defect. With heat stress, most ΔtpsAB spores were found to be non-viable, and those that were viable were severely delayed in germination, growth and subsequent sporulation. No clear correlation could be made between ΔtpsAB and susceptibility to oxidative stress; however ΔtpsAB may have an abnormal cell wall. Further studies in mice are underway to determine if the ΔtpsAB mutant is impaired in virulence, potentially making the trehalose synthase complex a promising new target for antifungal therapy.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.001 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".