Tethered To Telomeres: Characterizing The Form And Dynamic Elasticity Of Tethers In Anaphase Crane-Fly Spermatocytes
Bibliographic record
Abstract
Elastic tethers connect sister chromosomes during anaphase in a broad range of animal cells, likely aiding in communication and coordination of movement. Calyculin A prevents dephosphorylation of tethers by protein phosphatase 1 (PP1) and, consequently, causes them to remain elastic throughout anaphase, resulting in backward movement. In my thesis experiments, I further explore the composition and behaviour of tethers. I expand upon existing research with Calyculin A, elucidating an optimal working concentration of 10 nM for investigating the reversibility of this drug on backward movement when rinsed out at various stages. I show that PP1 is critical during the onset of anaphase and that backward movement is not readily reversible. I also support the existing notion that tethers are made of titin, disproving the hypothesis that tethers are made of DNA. These contributions further enhance our understanding of tether composition and regulation during a central mechanistic phase of cell division.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.003 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".