Electrophysiological assessment of chronic cytokine-mediated inflammation in rat ordanotypic hippocampal slice culture
Bibliographic record
Abstract
Chronic neuroinflammation was studied using whole-cell patch-clamp technique in rat organotypic hippocampal slice culture through exposure to lipopolysaccharide (LPS), an inflammatory stimulus that acts primarily by the induction of pro-inflammatory mediators known as cytokines. Seven-day exposure to LPS (100 ng/ml) resulted in significant changes in both intrinsic and synaptic properties of CA1 pyramidal neurons, which included decreases in membrane resistance and action potential (AP) discharge frequency; and potentiations of evoked monosynaptic excitatory post-synaptic current (EPSC) and inhibitory post-synaptic potential (IPSP) amplitudes. Changes in inhibitory synaptic transmission were suppressed when cultures were co-incubated with the interleukin-1 receptor antagonist (IL-1Ra, 50 ng/ml), suggesting that the cytokine IL-1 has an integral role in mediating this effect. Collectively, these data show that chronic inflammatory treatment produces important changes in CA1 neuronal properties, and that the effect on GABAergic transmission appears to be mediated by IL-1.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.001 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.001 |
| Insufficient payload (model declined to judge) | 0.003 | 0.001 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".