Substrate specificities of outer membrane proteases of the omptin family in «Escherichia coli, Salmonella enterica, and Citrobacter rodentium»
Bibliographic record
Abstract
Outer membrane proteins (OMPs) of the omptin family are present in some Gram-negative bacteria. Omptins protect cells by cleaving antimicrobial peptides (AMPs). For example, the Escherichia coli OmpT and OmpP impart resistance by cleaving the AMP protamine, and PgtE of Salmonella enterica was also reported to cleave AMPs. Despite identification of many in vitro substrates, few physiological substrates have been tested. Our laboratory recently identified the omptin CroP of Citrobacter rodentium, which cleaves the murine α-helical AMP mCRAMP and the similarly α-helical synthetic AMP C18G. We examined AMPs as substrates of several omptins. OmpT, OmpP, and CroP cleave the AMP C18G more effectively than PgtE, leading to higher minimal inhibitory concentrations (MICs). This suggests that AMP cleavage is an important function of OmpT, OmpP, and CroP during infection. Based on initial results, we attempted to compare and contrast specificity and catalytic activity of C18G cleavage by purified CroP and PgtE.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".