Structural and functional aspects of recombinant antibody fragment against bovine herpesvirus-1
Bibliographic record
Abstract
With the objective to construct single chain variable fragments (scFv) with enhanced ability to neutralize bovine herpes virus-1 (BoHV-1), V[lambda]J[lambda], and VHDHJ H recombinations isolated from anti-BoHV-1 IgG1 secreting hybridoma, were linked together by two amino acids. The V[lambda]J[lambda] -linker-VHDHJH amplicon was cloned into pPICZ[alpha] vector for expression in 'Pichia pastoris'. A recombinant scFv (scFv5-2L), secreted by 'P. pastoris' was characterized. The scFv5-2L had significantly higher BoHV-1 neutralization kinetics (p<0.001) 'in vitro' when compared to a scFv with an 18 amino acid linker (scFv3-18L). Such increased virus neutralization by scFv5-2L is likely due to higher avidity as a result of multimerization per se. The recombinant scFv recognized a viral neutralizing epitope on a 32 kDa envelope glycoprotein BoHV-1. In conclusion, the recombination scFv5-2L against BoHV-1 provides an improved potential diagnostic and therapeutic agent for the prevention and eradication of an economically important viral disease of cattle.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".