Structural and functional aspects of recombinant antibody fragment against bovine herpesvirus-1
Bibliographic record
Abstract
With the objective to construct single chain variable fragments (scFv) with enhanced ability to neutralize bovine herpes virus-1 (BoHV-1), V[lambda]J[lambda], and VHDHJ H recombinations isolated from anti-BoHV-1 IgG1 secreting hybridoma, were linked together by two amino acids. The V[lambda]J[lambda] -linker-VHDHJH amplicon was cloned into pPICZ[alpha] vector for expression in 'Pichia pastoris'. A recombinant scFv (scFv5-2L), secreted by 'P. pastoris' was characterized. The scFv5-2L had significantly higher BoHV-1 neutralization kinetics (p<0.001) 'in vitro' when compared to a scFv with an 18 amino acid linker (scFv3-18L). Such increased virus neutralization by scFv5-2L is likely due to higher avidity as a result of multimerization per se. The recombinant scFv recognized a viral neutralizing epitope on a 32 kDa envelope glycoprotein BoHV-1. In conclusion, the recombination scFv5-2L against BoHV-1 provides an improved potential diagnostic and therapeutic agent for the prevention and eradication of an economically important viral disease of cattle.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".