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Record W7054732723

AMPK mediated regulation of meiotic progression in «Caenorhabditis elegans»

2015· dissertation· no· W7054732723 on OpenAlexaff

Bibliographic record

VenueeScholarship@McGill (McGill) · 2015
Typedissertation
Languageno
FieldEngineering
TopicLaser Design and Applications
Canadian institutionsMcGill University
Fundersnot available
KeywordsAMPKCaenorhabditis elegansCell cycleStem cellProtein kinase ACell cycle checkpointGermlineFunction (biology)GeneGerm cell
DOInot available

Abstract

fetched live from OpenAlex

Cell cycle quiescence, a state of reversible arrest, is vital for the coordinated development of an organism and is a feature shared by many cell types, including stem cells.Suggested to play a role in protecting stem cells, it may also be adopted by cancer stem cells to their advantage.To enhance our knowledge on the regulation of cell cycle quiescence, we used the G2/M arrested germline of dauer Caenorhabditis elegans larvae as a model.AMP-activated kinase (AMPK) regulates the arrest of these germ cells, where compromised AMPK function leads to cell cycle progression.Using sensitized C. elegans dauer larvae, we employed a targeted RNAi-based screening approach to identify predicted AMPK phosphorylation targets involved in arresting meiotic progression.17 genes were identified, ranging in function from protein degradation, endogenous-siRNA pathways, and post transcriptional repression or activation.Further characterization of AMPK and its predicted targets will enhance our understanding of how meiotic progression and quiescence is regulated, in turn contributing to studies linking cell cycle quiescence and malignancies.fun, friend, leader, supervisor, Dr.Richard Roy. His enthusiasm for science has always been contagious and I am sincerely grateful for having worked in his lab.His assistance in helping me think critically about my project/science and

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.003
Threshold uncertainty score0.007

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0010.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.016
GPT teacher head0.252
Teacher spread0.235 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations0
Published2015
Admission routes1
Has abstractyes

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