Effects of di-(2-ethylhexyl) phthalate and four of its metabolites on steroidogenesis in MA-10 cells
Bibliographic record
Abstract
Plasticizers aid in processing, and impart flexibility to plastics. Their broad use and tendency to leach out of polymers have rendered them ubiquitous in the environment. Phthalate plasticizers, in particular di-(2-ethylhexyl) phthalate (DEHP), are known to cause male reproductive tract defects in animal models. It has been assumed that DEHP metabolite, mono-(2-ethylhexyl) phthalate (MEHP), is the active compound, however the bioactivity of metabolites such as 2-ethylhexanol, 2-ethylhexanal and 2-ethylhexanoic acid, has not been thoroughly investigated. The aim of this study was to test the effects of these compounds in a mouse Leydig tumour cell line, MA-10 cells. DEHP, MEHP and 2-ethylhexanal decreased cell viability, as well as steroidogenic potential as quantified by an enzyme-linked immunosorbent assay and gene expression analysis. Interestingly, 2-ethylhexanal was the most potent steroidogenic disruptor, which offered an intriguing contribution to the search for the mechanism(s) of phthalate toxicity and raised doubts that MEHP is the single active metabolite.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.002 | 0.001 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".