Effect of Adiponectin Overexpression on the Metabolic Phenotype of the «Ceacam1-/-mouse»
Bibliographic record
Abstract
CEACAM1 is a membrane glycoprotein expressed in many cells and tissues, amongst them liver and intestine. Ceacam1-/- mice are hyperinsulinemic and display certain MetS (metabolic syndrome) parameters. Adiponectin (Ad) is an insulin-sensitizing adipokine involved in MetS. Ceacam1-/- mice have increased tumour burden in models of intestinal carcinogenesis. OBJECTIVE: The goal of this project was to attempt to reverse the insulin resistance of Ceacam1-/- mice by overexpression of Ad, to quantify metabolic parameters in the different genotypes and to evaluate the suitability of the CC1 : Ad mouse model in studying the role of CEACAM1 insulin resistance in tumour progression. PROCEDURE: The Ad transgene was transferred from the FVB to the C57Bl/6 mouse background. Mice with different CC1 : Ad genotypes were produced. 6 month-old mice were bled and dissected after a five hour fast. Total body, fat pad (interscapular, inguinal, gonadal) and liver weights, as well as glucose, insulin and Ad levels were measured. RESULTS: The Ad transgene was correctly transferred from the FVB to the C57Bl/6 mouse background. Ad was similarly over-expressed and secreted. All parameters observed display comparable profiles between both strains, with slight differences in net levels induced by the susceptibility of the C57Bl/6 strain to insulin resistance. No difference between Ceacam1 wt and ko mice was observed for all parameters. However, some differences appear in presence of Ad overexpression, with CC1+/- : Ad tg+ and CC1-/- : Ad tg+ females being heavier than CC1+/+ : Ad tg+ females. CC1-/- : Ad tg+ males also have lower insulin and higher Ad levels than CC1+/+ : Ad tg+ males. DISCUSSION: The absence of differences between Ceacam1 wt and ko mice in the parameters examined in this study did not allow evaluation of the rescue by Ad. The rescue will now be evaluated for insulin resistance by the hyperinsulinemic euglycemic clamp technique, on the development of fatty l
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".