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Record W7057613268

KARAKTERISASI PROTEIN D-ENDOTOXIN DARI Bacillus thuringiensis SUBSPESIES Israelensis SEROTIPE H-14 SEBAGAI BIOINSEKTISIDAL LALAT

2008· other· id· W7057613268 on OpenAlexaboutno aff

Bibliographic record

VenueUniversitas Airlangga Repository (Universitas Airlangga) · 2008
Typeother
Languageid
FieldPhysics and Astronomy
TopicMagnetic confinement fusion research
Canadian institutionsnot available
Fundersnot available
KeywordsBacillus thuringiensisBacillus pumilusBacteriaBacillus (shape)
DOInot available

Abstract

fetched live from OpenAlex

Isolasi dan identifikasi Bacillus thuringiensis subspesies Israelensis serotipe H-14 bertujuan untuk memberikan informasi ilmiah tentang fraksi protein 8-endotoksin yang mampu berfungsi sebagai insektisida pada stadium larva dan dewasa dari beberapa spesies lalat. Isolat bakteri Bacillus thuringiensis subspesies. Israelensis serotipe H-14 dengan kode industri Bt. PS201L1 NRRL B-18750 dalam bentuk sheat kristal kering diperoleh dari Koppert Canada Limited. Bakteri selanjutnya perlu dibangkitkan aktivitasnya dan ditumbuhkan pada medium pertumbuhan. Koloni yang tumbuh kemudian diisolasi dan diperiksa di bawah mikroskop untuk memastikan bentuk bakteri. Untuk mengetahui aktifitas fungsional bakteri dilakukan uji biokimiawi dengan TSIA, SIM, Sitrat, Urease, MR/VP, Gula-gula, Glukosa, Laktosa, Maltosa, Manosa, Sukrosa, Dulsitol, Dekstrose, Raminose, Ratinose, Arabinose, Inulin, Salisin. Isolasi dan identifikasi protein bakteri dilakukan dengan mengambil dari koloni yang tumbuh pada medium biakan dan dimasukkan ke PBS, selanjutnya disentrifugasi dan substrat siap untuk diseparasi berdasarkan berat molekulnya dengan SDS-PAGE. Demikian pula dengan toksin yang disekresikan diambil dari medium cair, kemudian dilakukan presipitasi dengan Ammonium Sulfat jenuh sama banyak selama 24 jam pads suhu 4° C. Hasil yang diperoleh dari penelitian ini ditemukan berat molekul protein dari whole bakteri Bacillus thuringiensis subspesies israelinsis sebesar 103, 90, 78 dan 57 kDa dan berat molekul protein hasil presipitasi sebesar 103, 90, 78, 57, 53, 44, dan 33 kDa. Berat molekul protein tunggal 8-endotoksin sebesar 103 kDa yang diduga mempunyai aktifitas sebagai insektisidal alami.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.004
Threshold uncertainty score0.014

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0010.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0010.000
Open science0.0000.000
Research integrity0.0000.001
Insufficient payload (model declined to judge)0.0040.001

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.007
GPT teacher head0.185
Teacher spread0.178 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations0
Published2008
Admission routes1
Has abstractyes

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