Metabolic phenotyping of barley genotypes varying in resistance to Fusarium head blight
Bibliographic record
Abstract
Fusarium head blight (FHB), caused by Fusarium graminearum is a devastating disease of barley (Hordeum vulgare L.).Resistance in barley to FHB is quantitatively inherited and breeding for resistance is the most viable option to manage this disease.Though several FHB resistance quantitative trait loci (QTL) have been identified, the mechanism(s) of resistance is still unclear.A technology to uncover the mechanisms of resistance to FHB to aid in breeding for resistance is of immediate need for barley breeders.Metabolic profiling was applied to distinguish resistance in barley to FHB.This investigation reports three progressive studies.The first study explored the metabolic phenotyping of resistance in two barley recombinant inbred lines (RIL), H106-4 (resistant) and H106-371 (susceptible) against FHB.This study identified several resistance related (RR) metabolites.The second study was conducted to validate the RR metabolites identified in study-I, and also to identify novel RR metabolites.In study-II, five very resistant two-row genotypes, 'CIho4196', 'Zhedar-1', 'Zhedar-2','Fredrickson', 'Harbin-2r' and a susceptible CH9520-30 were phenotyped based on metabolic profiling.RR metabolites occurring in more than one genotype were considered potential biomarkers.In study-III, four RILs, two each of black and yellow barley, varying in resistance to FHB were phenotyped for resistance against trichothecene-producing and nonproducing (tri5 -) isolates of F. graminearum based on metabolic profiling.The metabolites were extracted from mock-inoculated or F. graminearuminoculated spikelets using aqueous methanol and analyzed using LC-ESI-LTQ-Orbitrap.Data was analyzed through XCMS and CAMERA bioinformatics tools.Compounds were identified based on accurate mass, fragmentation pattern and carbon-isotopic ratio.Thousands of peaks were detected, of which hundreds of treatment significant peaks were selected based on t-tests.These treatment significant metabolites were classified into resistance related constitutive (RRC) or induced (RRI).Barley resists FHB, through jasmonate-signaling and phenylpropanoids.Jasmonate was induced against trichothecene producing but not against the nonproducing isolate, tri5 -mutant.All barley genotypes (CEROM), Québec, Canada, who funded this work.
Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.
How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".