Regulation of cardiolipin biosynthesis by phospholipid scramblase-3
Bibliographic record
Abstract
Cardiotipin is an important phosphotipid synthesized in the inner mitochondrial membrane.lt is transported to the outer mitochondrial membrane by a newty described protein phosphotipid scrambtase-3 (PL53).The purpose of this project was to assess if biosynthesis of cardiolipin was inftuenced by varying the tevels of functionat PLS3.Hela celts expressing disrupted PL53 (F258V) or overexpressing PLS3 (PLSCR3) or control were incubated with [3H]gtycerol and incorporation of radioactivity into cardiotipin was determined.lncorporation of [3H]gtycerol into cardiotipin increased 2.1- fotd in F258V ce[[s and 1.8-fotd in PLSCR3 cetts compared to controt.This coincided with a 2.6-fotd increase in cardiotipin synthase activity in F258V cetts and a 1.ó-fotd increase in cardiotipÍn synthase activity in PLSCR3 cetls compared to controt.There was also a 0.78-fotd and 0.52-fotd increase in retative [eve[ of CL synthase RNA in F258V and PLSCR3 cetts, respectivety, compared to control.ln contrast, [14C]tinoteic acid incorporation into cardiotipin, a measure of cardiotipin remodeting, was reduced 0.45-fotd in F258V cetls and 0.52-fotd in PLSCR3 compared to controt.This was due to a decrease in mitochondrial monotysocardiotipin acyttransferase activity in both F258V and PLSCR3 cetts compared to controt.[3H]Serine incorporation into mìtochondrial phosphatidytethanotamine was unattered, indicating the effect of altered functional levets of PLS3 was exctusive to cardiotipin.ln summary, the data indicate that changes in cardiotipin synthesis may be tinked to altered II levets of PLS-3.Hence, PLS3 may be an important regulator of cardiotipin de novo biosynthesis, and an increase in cardiotipin biosynthesis may be a compensatory response to the rnodified trafficking of mitochondrial cardiotipin.
Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.
How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.001 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.001 |
| Insufficient payload (model declined to judge) | 0.002 | 0.001 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".