Analysis of indole-3-butyric acid auxin activity in Arabidopsis
Bibliographic record
Abstract
0-612-98352-8 L'auteur a accord une licence non exclusive permettant la Bibliothque et Archives Canada de reproduire, publier, archiver, sauvegarder, conserver, transmettre au public par tlcommunication ou par l'Internet, prter, distribuer et vendre des thses partout dans le monde, des fins commerciales ou autres, sur support microforme, papier, lectronique et/ou autres formats.L'auteur conserve la proprit du droit d'auteur et des droits moraux qui protge cette thse.Ni la thse ni des extraits substantiels de celle-ci ne doivent tre imprims ou autrement reproduits sans son autorisation.Conformment la loi canadienne sur la protection de la vie prive, quelques formulaires secondaires ont t enlevs de cette thse.Bien que ces formulaires aient inclus dans la pagination, il n'y aura aucun contenu manquant. Abstrad:Auxins are plant hormones involved in virtually all aspects of plant life.Despite long-term commercial and horticultural use of the auxin Indole-3-Butyric Acid (IBA), a fun recognition of its natural occurrence in plants was made only recently. 1 have used multiple approaches to dissect the role of IBA in Arabidapsis thaliana.This thesis includes the first characterization of a mutant with an altered response to IBA that retains wild-type sensitivity to Indole-3-Acetic Acid (lAA), the most studied endogenous auxin.This mutant, named resistant ta IRA (ribl), has modified root architecture and gravitropism and is resistant to auxin transport inhibitors.As the se phenotypes are reminiscent of those of characterized auxin transport mutants, movement of IAA and IBA was studied in wild-type and mutant plants.IBA is transported in seedlings in three distinct flows, like IAA, and this transport is saturable, indicating it is carrier mediated.However, unlike IAA, IBA is not polarly transported in inflorescence axes, and IBA transport is not sensitive to IAA transport inhibitors.These results suggest IAA and IBA transport could be mediated or regulated by different mechanisms.In ribl seedIings, an flows of IBA transport are modified, while IAA transport levels are unchanged.Modifications in IBA transport match phenotypic differences between ribl and wild-type, and analyses of the physiological effects of IBA also suggest IBA has a role in defining wild-type seedIing morphology in Arabidopsis.Though IAA transport levels are not changed in ribl, one flow of IAA transport is rendered insensitive to IAA transport inhibitors, perhaps revealing cross-talk between IAA and IBA transport regulation.AdditionaHy, double mutant analyses reveal that IAA transport and response mutants can suppress sorne phenotypes of ribl, and sorne mutant combinations produce novel phenotypes, further suggesting cross-talk between IBA and IAA transport and response pathways.Finally, l found that the light signaling hy5 mutant shares many of the same phenotypes as ribl and double mutant analysis suggests HY5 could be partially redundant with RIB 1 in regulating many of these.More complete analysis of double mutants could further define pathways in which RIB l, and IBA transport, play an important roie. RsumLes auxines sont des hormones vgtales impliques dans presque tous les aspects de la vie de la plante.Bien que l'acide indole-3-butyrique (AIB) soit utilise depuis longtemps des fins commerciales et horticoles, sa prsence comme auxine endogne des plantes n'a t pleinement reconnue que rcemment.J'ai utilis plusieurs approches afin de dfinir le rle de rAID chez Arabidopsis thaliana.Cette thse contient la premire caractrisation d'un mutant prsentant des dfauts de rponse l'AIB, tout en conservant une rponse normale l'acide indole-3-actique (AIA), l'auxine endogne la plus tudie.Ce mutant, nomm resistant to IBAl (abbrvi ribl), prsente une architecture et un gravitropisme des racines modifis et est rsistant aux inhibiteurs de transport de l'AIA.Comme ces phnotypes rappellent ceux de mutants bien caractriss de transport d'auxine, nous avons tudi le transport de l'AIA et de l'AIB chez des plantes mutantes ribl et de type sauvage.Tout comme l'AIA, l'AIB est transport dans les plantules via trois flux distincts.Ce transport est saturable, indiquant qu'il est mdi par des
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.001 | 0.001 |
| Meta-epidemiology (broad) | 0.001 | 0.001 |
| Bibliometrics | 0.002 | 0.004 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.001 |
| Open science | 0.001 | 0.000 |
| Research integrity | 0.001 | 0.001 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".