A Campylobacter jejuni glycosylation island important in cell charge, legionaminic acid biosynthesis and colonisation of chickens
Bibliographic record
Abstract
Previously we identified five genes (Cj1321-26) in the O-linked flagellin glycosylation island that were highly prevalent in Campylobacter jejuni isolates from chickens. We report mutagenesis, functional and structural data to confirm that this locus, and Cj1324 in particular, has a significant contributory role in the colonisation of C. jejuni in chickens. A motile DeltaCj1324 mutant with intact flagellae was considerably less hydrophobic and less able to autoagglutinate and form biofilms than the parent strain 11168H, suggesting that the surface charge of flagellae was altered in Cj1324-deficient strains. These physical and functional attributes were restored upon complementation. Structural analysis of flagellin protein purified from the DeltaCj1324 mutant revealed the absence of two legionaminic acid glycan modifications that were present in the parent strain 11168H. These glycoform modifications were shown to be prevalent in chicken isolates and confirm that differences in the highly variable flagellin glycosylation locus can relate to strain source. The discovery of molecular mechanisms influencing persistence of C. jejuni in poultry aids the rational design of approaches to control this problematic pathogen in the food chain
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".