Exploration of «Ac/Ds» transposons in wild barley, «Hordeum vulgare» L. subsp. «spontaneum» (C. Koch) Thell.
Bibliographic record
Abstract
Domestication and intensive selective breeding of cultivated plants has triggered erosion of genetic diversity of important stress related alleles.Researchers highlight the potential of using wild accessions as a gene source for improvement of cereals such as barley, which has major economic and social importance worldwide.Based on the introduction of the maize Ac/Ds transposon system for gene identification in cultivated barley, the objective of this research is to investigate the response of Hordeum spontaneum accessions in tissue culture to standardize parameters for introduction of Ac/Ds transposons into the wild barley genome through genetic transformation.We investigated the response of 10 wild barley genotypes for callus induction, regenerative green callus induction and regeneration of fertile plants.The activity of exogenous Ac/Ds elements was observed through a transient assay on immature wild barley embryos/callus.Transformed calli were identified using Hygromycin B as a selectable agent in the media and by the expression of GFP.Forty six bombardment experiments were performed on 3952 pieces of callus (3-5 mm each) in 10 genotypes of wild barley.The transformation frequency of putative transgenic callus lines based on Hygromycin B selection and the GFP reporter system ranged from 0-25.7% in wild barley genotypes.Five hundred and five putative independent transgenic callus lines were generated in this study, from which 54 plants were regenerated.Initial molecular screening analysis for Ac/Ds transposon insertions on a subset of regenerated plants confirmed the transgenic status of wild barley lines.This is the first report of a transformation system in H. spontaneum.VIII
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.001 |
| Insufficient payload (model declined to judge) | 0.003 | 0.001 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".