Functional analysis of «Haemonchus contortus» P-glycoprotein-A and interaction with macrocyclic lactones
Bibliographic record
Abstract
Macrocyclic lactone (ML) resistance has been described in the parasitic nematode, Haemonchus contortus. One of the mechanisms involved could be the over expression of P-glycoproteins (Pgps) which are ABC transporters. These proteins may influence the concentration of MLs that reach their target. In H. contortus one ABC transporter that is overexpressed in ML resistant parasites is Pgp-A (HcPgp-A). The goal of this project was the expression of HcPgp-A, in transfected LLC-PK1 cells, and to see the effect of ivermectin and moxidectin on inhibition of rhodamine 123 transport by the transfected cells. Rhodamine123 was actively transported by HcPgp-A. Ivermectin was four fold more potent at inhibiting rhodamine 123 transport by HcPgp-A than was moxidectin. The work provides the first information showing that MLs can inhibit the transport of Pgp substrates by a parasitic nematode ABC transporter and may indicate an active role for H. contortus Ppgs in ML resistance.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".