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Record W7066041187

The Genetic Basis of Natural Variation in Algal Neutral Lipid Accumulation

2021· dissertation· W7066041187 on OpenAlexfundno aff

Bibliographic record

VenueTSpace · 2021
Typedissertation
Language
FieldEnergy
TopicAlgal biology and biofuel production
Canadian institutionsnot available
FundersUniversity of Toronto
KeywordsChlamydomonas reinhardtiiIntraspecific competitionGenetic variationAdaptation (eye)Natural selectionAlgaeSelection (genetic algorithm)NutrientLatitude
DOInot available

Abstract

fetched live from OpenAlex

Neutral lipids are known to be involved in an algal adaptation to cold, light, and nutrient stress, but the extent of intraspecific variation and the mechanisms maintaining the variation in this economically important trait are unclear. I predict algal strains from different latitudes will vary in neutral lipid accumulation because many of the stressor associate with climate. Inducing neutral lipid accumulation using nitrogen (N) starvation in 26 natural isolates of Chlamydomonas reinhardtii revealed genetic variation but no latitudinal pattern in neutral lipid accumulation. A further experiment demonstrated latitudinal variation in neutral lipid accumulation induced by cold shock and light deprivation. By analyzing molecular evolution of 473 lipid-candidate genes, I inferred stronger purifying selection and higher rates of positive selection driving genetic divergence in the lipid-associated genes. From this, I suggest the possibility that neutral lipid accumulation is an adaptive trait in C. reinhardtii under positive selection.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.001
Threshold uncertainty score0.003

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0010.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.020
GPT teacher head0.313
Teacher spread0.293 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations0
Published2021
Admission routes1
Has abstractyes

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