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Record W7066705738

The involvement of phospholipase A₂ (PLA₂ ) in acylation stimulating protein (ASP) signaling /

2005· dissertation· en· W7066705738 on OpenAlexaboutno aff

Bibliographic record

VenueeScholarship@McGill (McGill) · 2005
Typedissertation
Languageen
FieldBiochemistry, Genetics and Molecular Biology
TopicProtein Kinase Regulation and GTPase Signaling
Canadian institutionsnot available
Fundersnot available
KeywordsPhospholipase CSignal transductionEnzymePhospholipasePhospholipase A
DOInot available

Abstract

fetched live from OpenAlex

Preliminary data suggests that the lipogenic factor Acylation Stimulating Protein (ASP), stimulates the activity of calcium-dependent phospholipase A2 (cPLA2) by increasing intracellular calcium levels [Ca2+]i and by activating extracellular-signal-regulated kinase 1/2 (ERK 1/2). The arachidonic acid (AA) generated by cPLA2 action appears to function as a second messenger in ASP signaling. ASP also blocks TG breakdown. The calcium-independent PLA2 (iPLA2) zeta has recently been identified as a novel TG-lipase in 3T3-L1 cells. Bromoenol lactone (BEL), a non-reversible iPLA2 inhibitor, has been shown to specifically inhibit the TG-lipase activity of this enzyme. Preliminary data demonstrates that BEL stimulates basal TG synthesis, likely by inhibiting TG breakdown. The effects of BEL in combination with ASP are non-additive, suggesting they act through the same pathway. Furthermore, ASP appears to inhibit 3H-AA release into the media in a concentration-dependent manner. We propose that ASP inhibits an iPLA2 isoform with TG-lipase activity, an effect that can be mimicked by BEL.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.005
Threshold uncertainty score0.018

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0010.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0050.002

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.013
GPT teacher head0.249
Teacher spread0.236 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations0
Published2005
Admission routes1
Has abstractyes

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