Moderate vitamin B6 deficiency and sulfur amino acid metabolism in male and female rats
Bibliographic record
Abstract
Homocysteine, cysteine, and methionine are sulfur amino acids. Methionine converts to cysteine in the transsulfuration pathway, utilizing homocysteine as an intermediate. Research indicates that vitamin B6 deficiency may impair the regulation of cellular homocysteine concentrations and decrease cysteine synthesis. Previous studies on B6 deficiency focused solely on male rats overlooking sex-specific differences. This study aims to demonstrate that moderate vitamin B6 deficiency intake in rats affects methionine plasma levels, leading to reduced conversion into cysteine within the transsulfuration pathway compared to the adequate vitamin B6 group. Thus, moderately deficient rats are expected to exhibit decreased B6 plasma levels, with sex contributing to specific differences. Forty-six seven-week-old female and male Sprague-Dawley rats were randomly assigned to receive either a moderate (0.7 mg/day) or adequate (7.0 mg/day) B6 diet for five weeks. Plasma SAA, B6, estrogen, progesterone, and 1-carbon metabolites in plasma, and enzyme expression of CBS and CGL in the liver were determined. A significant diet effect was observed in 5-MTHF, GSH, choline, glycine, and cystine, with lower plasma levels in the moderate B6 diet group. Conversely, cystathionine and GSSG showed higher plasma levels in the same diet group. Furthermore, a sex effect was evident, with significantly higher plasma levels in male groups for 5-MTHF, methionine, DHFR, vitamin B12 and B2, acetylcholine, choline glutamic acid, SAM, glycine, serine, and GSSG. On the contrary, total cysteine, estrogen, and betaine were significantly higher in female groups. Finally, a sex-by-diet interaction was observed in CBS enzyme expression, with males exhibiting higher expression levels in the adequate diet group compared to the moderate deficiency group, while females showed higher expression levels in the moderate B6 deficient diet group. This study underscores the importance of including both sexes in nutrition research and emphasizes the importance of maintaining an adequate intake of B6 to support optimal functioning of the transsulfuration pathway and metabolism.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.001 | 0.001 |
| Meta-epidemiology (broad) | 0.001 | 0.001 |
| Bibliometrics | 0.002 | 0.000 |
| Science and technology studies | 0.001 | 0.001 |
| Scholarly communication | 0.000 | 0.001 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.001 | 0.001 |
| Insufficient payload (model declined to judge) | 0.003 | 0.001 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".