Identifying fungal associations of the endangered plant Crocanthemum canadense (L.) Britt. (Cistaceae) in Nova Scotia
Bibliographic record
Abstract
Crocanthemum canadense (L.) Britt. (Cistaceae) is a small, herbaceous plant that is critically imperiled in Nova Scotia. Habitat loss and an insect pest, Mompha capella (Momphidae), are contributing to population decline. The role of fungal associations in C. canadense populations is unknown, and thus the primary goal of this research was to identify fungal symbionts to aid in propagation and conservation strategies. Fungi were isolated from the roots, stems, and leaves of C. canadense plants collected throughout six sites at 14 Wing Canadian Forces Base Greenwood, Nova Scotia. Arbuscular mycorrhizal fungi (AMF) were observed using root staining and microscopic techniques to determine percent root colonization. Site 3 had the highest average A MF colonization rate (53%; n = 3). Using ITS rDNA barcoding, 43 fungal species were identified from roots, 14 from leaves, 6 from upper stems, and 11 from lower stems. Six ascomycete fungi occurred in more than half of the study sites and colonized more than a third of the plants sampled. These included Pyrenophora tetrarrhenae colonizing 89% of plants which was isolated from above-ground tissues, Arcopilus aureus (56%; from all tissue types), Pilidium concavum (50%; from roots, leaves, and lower stems), Phialocephala cf. fortinii (44%; from roots and lower stems), Apiognomonia cf. hystrix (39%; from all tissue types), and Sphaeronaemella fragariae (39%; only from leaves). Apiognomonia cf. hystrix and P. tetrarrhenae were present in C. canadense tissue at all six sites. This research provides the first report of the fungal relationships potentially influencing endangered Nova Scotia C. canadense populations and could be used to enhance the success of future plant propagation and conservation efforts.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.001 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".