Interleukin 7 requirement for survival of T-cell
Bibliographic record
Abstract
acute lymphoblastic leukemia and human thymocytes on bone marrow stroma We explored the role of interleukin-7 (IL-7) in the bone marrow (BM) stroma-mediated survival of primary T-cell acute lymphoblastic leukemia (T-ALL) cells and normal thymocytes. We present evi-dence that IL-7 has a major role in the enhanced survival mediated by BM stroma both in T-ALL cells and thymocytes. Haematologica 2007; 92: 264-266 T-cell acute lymphoblastic leukemia (T-ALL) cells and thymocytes, their normal counterpart, undergo sponta-neous apoptosis when cultured in vitro. Co-cultures with bone marrow (BM)1,2 or thymic stroma3 can induce T-ALL survival and proliferation. However, the molecular mech-anisms promoting these effects in the microenvironments remain poorly elucidated. Interleukin-7 (IL-7), produced by BM and thymic stroma, plays a crucial role in the development of normal T cells4 and contributes to the pathogenesis of T-cell leukemia.5 IL-7 induces survival6 and proliferation of early thymocytes7 and regulates sur-vival, cell cycle, and growth of primary T-ALL cells.5 To explore the mechanisms involved in the enhanced survival mediated by BM stroma, we cultured primary T-ALL cells or normal human thymocytes in vitrowith either human BM stromal cells obtained from seven healthy donors, as previously described,8 or the murine M2-10B4 fibroblast-like cell line of BM stromal origin (kindly pro-vided by Dr Connie J Eaves, Terry Fox Laboratories, Vancouver, Canada). T-ALL cells were derived from enriched leukemic cells isolated from adult patients and classified according to their maturation stage by immunophenotypic analysis (Table 1), as previously described.9 T-ALL cells were considered positive for a defined antigen if at least 30 % of cells were positive com-pared to the isotype-matched control. Thymocytes were derived from normal thymuses of children (<5 years of age) undergoing cardiac surgery. BM stroma induced a sig-nificant increase of survival in both T-ALL cells and thy-mocytes (Figure 1A and 1B), as measured by annexin V-fluoroscein isothiocyanate/propidium iodide staining
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.002 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".