Improved Efficiency of Plant Regeneration from Smooth Bromegrass (Bromus inermis) Suspension Culture
Bibliographic record
Abstract
Smooth bromegrass, Bromus inermis Leyss, is the most important forage grass in Canada. Breeding for improvements such as higher yield and faster regrowth has made little progress, mainly due to the polyploidy and cross-pollination which are characteristic of this grass. The application of tissue culture methods to bromegrass improvement holds promise only if an efficient procedure for plant regeneration is found. Various basal culture media and auxins were tested for their effects on callus induction, growth of suspension cultures and plant regeneration. None of the tested auxins gave better in vitro growth than 2,4-D. Eriksson's medium with 2,4-D as the sole plant growth regulator was superior to all other tested media in suspension culture growth rate. The highest frequency of plant regeneration was obtained using the following regime: 1) growing suspension culture in Eriksson's medium supplemented with 2.5 µ.m 2,4-D, and 2) plating cell aggregates on hormone-free Kao's B medium containing 8 g/1 agar. Addition of 50 mM proline or glutamine to any of the regeneration media was inhibitory to plant regeneration.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.001 | 0.000 |
| Meta-epidemiology (broad) | 0.001 | 0.000 |
| Bibliometrics | 0.001 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.001 |
| Research integrity | 0.000 | 0.001 |
| Insufficient payload (model declined to judge) | 0.001 | 0.001 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".