Functional roles of arabidopsis elongin A and C in UV stress tolerance and development
Why this work is in the frame
A frame that forgets how it found something cannot be audited. These are the routes that admitted this work.
Bibliographic record
Abstract
Elongins A (ELOA), B (ELOB), and C (ELOC) were originally identified in mammals as transcription elongation complex. Beyond this role, the three mammalian elongins function in E3 ligase complex that degrades the large subunit of the stalled RNA polymerase II after DNA damage. S. cerevisiae lacks ELOB but posseses ELOA and ELOC homologs which form a similar E3 ligase complex that performs a comparable function in targeting stalled RNA polymerase II for degradation. These yeast homologs are functionally conserved with their mammalian counterparts, as yeast ELOC enhances mammalian ELOA transcript elongation via interacting with it. However, yeast ELOA/C complex alone does not promote yeast transcriptional elongation. Their role in RNA polymerase II polyubiquitylation and degradation following DNA damage has been well characterized. In this study, we identified Arabidopsis elongin A homologue (ELOA; At2g42780) and examined the roles of ELOA and elongin C homologue (ELOC; At5g59140) in UV tolerance and development using molecular and genetic analyses. YFP-ELOA localized to nucleus and this pattern was not affected after UV treatment. However, YFP-ELOC localized to both nuclei and cytosol with reduced cytoplasmic signal post-UV exposure. Both Ateloa-2 and Ateloc-3 mutants exhibited enhanced UV-sensitive phenotypes in seedlings and adults. ELOA overexpression enhanced hypocotyl UV tolerance. Yeast two-hybrid assay confirmed the interaction between Arabidopsis ELOA and ELOC. Interestingly, ELOA and ELOC overexpression increased silique length and seed number, and ELOC overexpression also increased plant height. These findings demonstrate that Arabidopsis ELOA and ELOC homologs are required for UV tolerance and contribute to plant developmental regulation.
Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it