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Record W7116674959 · doi:10.1016/j.xcrm.2025.102517

In mouse and in vitro models, bowel preparation promotes pathogen colonization, translocation, and exacerbation of inflammation

2025· article· en· W7116674959 on OpenAlexafffund
Charlotte A Clayton, Imogen Porter, Bohan Deng, Giselle McCallum, Apsara Srinivas, Claire Sie, Jerry He, Alexander Pei, Dominique Tertigas, Deanna M. Pepin, Touran Fardeen, Katharine M. Ng, S Sinha, Michael G. Surette, Bruce A. Vallance, Carolina Tropini

Bibliographic record

VenueCell Reports Medicine · 2025
Typearticle
Languageen
FieldMedicine
TopicColorectal Cancer Screening and Detection
Canadian institutionsBC Children's HospitalMcMaster UniversityGenome British ColumbiaCanadian Institute for Advanced ResearchUniversity of British Columbia
FundersCanadian Institutes of Health ResearchCrohn's and Colitis CanadaUniversity of British ColumbiaCanada Excellence Research Chairs, Government of CanadaW. Garfield Weston FoundationAllen FoundationMichael Smith Health Research BCCanada Foundation for InnovationCanadian Institute for Advanced Research
KeywordsUlcerative colitisInflammatory bowel diseaseIn vitroColitisInflammationExacerbationBowel preparationPathogen

Abstract

fetched live from OpenAlex

In the United States, an estimated 14 million colonoscopies are performed yearly, each requiring patients to undergo bowel preparation, a laxative cleansing of the intestine's luminal contents. Despite its widespread use, the effects of bowel preparation on gut physiology and susceptibility to pathogens remain poorly understood, particularly in individuals with compromised gut health. Using mouse and in vitro models, we find that bowel preparation with the laxative polyethylene glycol rapidly disrupts the gut, transiently increasing susceptibility to infection by Salmonella Typhimurium, including a non-motile mutant, and by gut pathobionts derived from ulcerative colitis microbiota. Bowel preparation also facilitates bacterial translocation to extraintestinal sites (mesenteric lymph nodes, liver, and spleen) and exacerbates inflammation in a chemically induced colitis model. Although these findings are preclinical, they suggest that bowel preparation may have underappreciated risks in vulnerable populations and warrant further clinical investigation.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame distilled prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.

metaresearch head score (Codex)0.001
metaresearch head score (Gemma)0.000
Version: codex-gemma-dda1882f352aValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.127
Threshold uncertainty score0.328

Codex and Gemma teacher scores by category

CategoryCodexGemma
Metaresearch0.0010.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0000.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.010
GPT teacher head0.255
Teacher spread0.245 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one teacher head, not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations0
Published2025
Admission routes2
Has abstractyes

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