Sex differences in tau and synaptic pathologies in a humanized MAPT mouse model revealed by PET imaging
Bibliographic record
Abstract
Abstract Background Tauopathy is a prominent feature of dementia and is present in over 20 disorders including Alzheimer’s disease (AD) and frontotemporal dementia (FTD), involving the hyperphosphorylation and aggregation of microtubule associated protein tau (MAPT). A humanized knock‐in tauopathy mouse model with FTD‐related mutations, MAPT 10+3/S305N (S305N), presents with toxic 4‐repeat‐(4R) tau isoforms, phosphorylated‐tau inclusions, and synaptic degeneration. Our objective is to conduct PET imaging of female and male S305N mice for tau and synaptic pathologies, with [ 18 F]OXD‐2314 and [ 18 F]SynVesT‐1, respectively, and relate these findings to the behavioral and pathological phenotype to understand tauopathy progression, and potential sex differences. Method S305N mice were compared to non‐mutated MAPT knock‐in mice. Mice underwent PET/CT imaging with [ 18 F]OXD‐2314, advantageous to image mixed 3R/4R tau isoforms and non‐AD tau species, and [ 18 F]SynVesT‐1 for synaptic vesicle glycoprotein 2A. Brain region time activity curves (TACs) were extracted in standardized uptake values (SUV). Cognition was assessed in the Barnes maze, and sleep activity patterns by home‐cage video recording. Mouse brains were collected for immunohistochemical analysis of phosphorylated‐tau (PHF1, CP13). Result [ 18 F]OXD‐2314 SUVR 30‐60min (cerebellum as reference region) was significantly increased in the whole brain of female S305N mice, compared to female MAPT, notably in the frontal cortex with a trend in the hippocampus, and unchanged in the entorhinal cortex. No differences in [ 18 F]OXD‐2314 uptake were detected between males. [ 18 F]SynVesT‐1 demonstrated significantly reduced whole brain SUV 0‐90min and volume of distribution in female S305N mice compared to MAPT indicative of synaptic loss, but no changes were seen in males. Cognition and sleep were impacted in both sexes of S305N mice; yet cognitive deficits were more advanced in male S305Ns. Immunohistochemistry indicated increased tau aggregates in S305N mice in the frontal cortex, hippocampus and entorhinal cortex, with no sex differences. Conclusion PET imaging revealed more rapid tau and synaptic pathologies in female S305Ns despite greater cognitive deficits in male S305N mice. Pathological assessment for neurodegeneration is ongoing. Further characterization of [ 18 F]OXD‐2314 and [ 18 F]SynVesT‐1 in tauopathy mouse lines will support our PET imaging studies with these radiopharmaceuticals in patients with tauopathy.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.001 | 0.000 |
| Meta-epidemiology (narrow) | 0.001 | 0.000 |
| Meta-epidemiology (broad) | 0.001 | 0.001 |
| Bibliometrics | 0.002 | 0.000 |
| Science and technology studies | 0.000 | 0.001 |
| Scholarly communication | 0.001 | 0.001 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.001 | 0.001 |
| Insufficient payload (model declined to judge) | 0.004 | 0.001 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".