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Record W7117321624 · doi:10.64898/2025.12.24.696334

Lowering insulin mitigates female reproductive aging and diet-induced ovarian dysfunction

2025· article· W7117321624 on OpenAlexafffund
Faria Athar, Liam G. Hall, Xiaoke Hu, James D. Johnson, Nicole M. Templeman

Bibliographic record

VenuebioRxiv (Cold Spring Harbor Laboratory) · 2025
Typearticle
Language
FieldMedicine
TopicOvarian function and disorders
Canadian institutionsUniversity of British ColumbiaUniversity of Victoria
FundersCanadian Institutes of Health ResearchBanting Research Foundation
KeywordsHyperinsulinemiaInsulinInsulin responseOvarian reserveHyperinsulinismMenopauseGlucose tolerance testPancreatic hormone

Abstract

fetched live from OpenAlex

Abstract Hyperinsulinemia has consequences beyond metabolic dysfunction, including reproductive system effects. We found that hyperinsulinemia at age 46-47 was predictive of earlier menopause in the Study of Women’s Health Across the Nation. To test causality between insulin levels and reproductive aging, we longitudinally evaluated chow- or high-fat, high-sucrose (HFHS)-fed Ins1 -null female mice with full or partial Ins2 insulin gene expression. Ins1 -/- ;Ins2 +/- mice had lower HFHS-induced hyperinsulinemia and less weight gain than their full- Ins2 littermates, despite comparable HFHS-induced glucose intolerance up to 9 months. By 15 months, Ins1 -/- ;Ins2 +/+ ovaries showed multinucleated giant cell accumulation with HFHS, while Ins1 -/- ;Ins2 +/- mice were protected against this response and maintained a higher reserve of follicles. Moreover, aged Ins1 -/- ;Ins2 +/- mice were 9-fold more likely to conceive on HFHS than hyperinsulinemic Ins1 -/- ;Ins2 +/+ mice. Elevated insulin is therefore a critical mechanistic link between metabolic dysfunction and reproductive aging, and curtailing insulin levels protects against subfertility and HFHS-induced ovarian decline.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.002
Threshold uncertainty score0.006

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.001
Insufficient payload (model declined to judge)0.0020.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.011
GPT teacher head0.225
Teacher spread0.214 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations0
Published2025
Admission routes2
Has abstractyes

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