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Record W7117651683 · doi:10.1021/acs.jproteome.5c00694

Seasonal Changes in the Seminal Plasma Proteome of the Crab-Eating Fox ( <i>Cerdocyon thous</i> )

2025· article· en· W7117651683 on OpenAlexaff
Jaqueline Cândido de Carvalho, Marcos Gomides Carvalho, Viviane Maria Codognoto, Lisa Camargo, R. Kasimanickam, John P. Kastelic, Fabiana Ferreira de Souza, João Carlos Pinheiro Ferreira

Bibliographic record

VenueJournal of Proteome Research · 2025
Typearticle
Languageen
FieldBiochemistry, Genetics and Molecular Biology
TopicReproductive biology and impacts on aquatic species
Canadian institutionsUniversity of Calgary
FundersDivision of Graduate EducationCentro Nacional de Pesquisa em Energia e MateriaisCoordenação de Aperfeiçoamento de Pessoal de Nível Superior
KeywordsProteomeSemenProteomicsReproductionSeasonal breederBlood proteinsSpermReproductive biology

Abstract

fetched live from OpenAlex

). Semen was collected in Brazil from March 2021 to March 2022 from five healthy adult males housed individually. Collections were performed without chemical or physical restraint by digital manipulation of the penis, and seminal plasma proteomics were assessed by mass spectrometry (ESI Q-Tof MS/MS) on 43 ejaculates from the reproductive season and four from the nonreproductive season. A total of 408 proteins were identified: 219 exclusives to the reproductive season (June-September), 90 to the nonreproductive season (October-May), and 99 shared between both. Protein abundance differed significantly between seasons. Proteins related to enzymatic and oxidoreductase functions predominated in the nonreproductive season, whereas those linked to sperm metabolism and reproductive processes were more abundant in the reproductive season. Among these, olfactory receptor, strawberry notch homologue, and zinc finger protein were considered potential reproductive season biomarkers, with AUC > 0.80 in the receiver operating characteristic analysis. This is the first study describing the seminal plasma proteome and its seasonal variation in the crab-eating fox, identifying biomarkers with potential applications in conservation and reproductive management of this and other endangered canids.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame distilled prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.

metaresearch head score (Codex)0.005
metaresearch head score (Gemma)0.003
Version: codex-gemma-dda1882f352aValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.084
Threshold uncertainty score0.376

Codex and Gemma teacher scores by category

CategoryCodexGemma
Metaresearch0.0050.003
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.001
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0010.000
Research integrity0.0000.001
Insufficient payload (model declined to judge)0.0000.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.039
GPT teacher head0.370
Teacher spread0.331 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one teacher head, not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations0
Published2025
Admission routes1
Has abstractyes

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