Bibliographic record
Abstract
Neuronal calcium sensor-1 (NCS-1) belongs to a family of Ca2+ sensor proteins which is conserved from yeast to humans. Its originally identified homologue frequenin enhances synaptic efficacy at the neuromuscular junction in Drosophila and Xenopus and initial studies in neuroendocrine (PC12) cells also implicate NCS-1 in increasing Ca2+-dependent exocytosis. Its homologue in yeast, frg1, has been shown to be an essential gene, as the null mutant is lethal. Genetic screens have identified a phosphatidylinositol-4-kinase, pi1kp, as a specific suppressor. Frq1 physically interacts with pi1kp and upregulates its activity suggesting a distinct role of frg1 in modulating phosphatidylinositol-dependent signaling pathways. In this thesis, the role of NCS-1 in synapse formation and in transmitter release was examined in neuroblastoma (NG108)-myocyte co-cultures. Endogenous NCS-1 partially localizes with SNAP-25 and overexpression of NCS-1 in differentiated NG108 cells facilitates synapse formation and increases the quantal content of evoked aceytlcholine release. Stable PC12 cells overexpressing NCS-1 were used to examine its localization and role in Ca2+ signaling and exocytosis. NCS-1 is found in the light synaptic vesicle fraction showing a parallel distribution with synaptophysin. Both the potency of and magnitude of uridine 5′-triphosphate (UTP) or bradykinin-evoked increases in intracellular Ca2+ concentrations were enhanced in NCS-1 overexpressing cells. This enhancement correlated with increased UTP-stimulated phosphoinositide turnover. In addition, exocytosis evoked by UTP, but not by high K+, was enhanced by NCS-overexpression. These data indicate that NCS-1 regulates phosphatidylinositol turnover leading to enhanced Ca2+ signaling and exocytosis. In conclusion, these results demonstrate a role for NCS-1 in synaptic transmission and transmitter release in mammalian cell lines by regulating phosphatidylinositol-dependent signaling pathways.
Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.
How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.001 | 0.001 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.001 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".