Establishing a superior tetracycline-inducible gene expression system for mouse CD200, a potential therapeutic immunosuppressive molecule
Bibliographic record
Abstract
CD200 (originally called OX2) is a membrane glycoprotein expressed by a variety of cell types, including lymphocytes, neurons and endothelium. It is the ligand for CD200 receptors (CD200Rs) whose expression is limited to hematopoietic cells, especially myeloid cells. A number of in vivo studies have implied that the interaction of CD200 with CD200R1 may inhibit a variety of immune responses, including graft rejection, autoimmune disease, fetal loss syndrome, and cancer. To study further the biological function of CD200 and the CD200Rs, our lab produced TRE-CD200-EGFP transgenic mice using a Tet-On inducible system. This thesis evaluated the inducible function of rtTA2s-M2, a new generation of Tet-On regulator, and compared it to the old version of rtTA in vitro and in vivo. The data suggest that induction of the CD200-EGFP transgene in rtTA2s-M2 transgenic mice is superior to that achieved in rtTA transgenic mice following doxycycline feeding in vivo.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.001 |
| Insufficient payload (model declined to judge) | 0.002 | 0.001 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".