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Record W7132875180

Studies of autocrine VEGF signalling in rhabdomyosarcoma

2004· dissertation· W7132875180 on OpenAlexfundno aff
Matthew Frederick William Gee

Bibliographic record

VenueTSpace · 2004
Typedissertation
Language
FieldBiochemistry, Genetics and Molecular Biology
TopicAngiogenesis and VEGF in Cancer
Canadian institutionsnot available
FundersHospital for Sick Children
KeywordsAutocrine signallingRhabdomyosarcomaAngiogenesisVascular endothelial growth factorParacrine signallingRegulatorReceptor tyrosine kinaseTyrosine kinaseRetinoic acid
DOInot available

Abstract

fetched live from OpenAlex

Vascular endothelial growth factor (VEGF), a major regulator of angiogenesis and tumour growth, exerts signals through two receptor tyrosine kinases, VEGFR1 and VEGFR2. Many tumours cells also express VEGF receptors and are influenced by autocrine VEGF signalling. Expression of VEGFR1 is induced in cells with a PAX3-FKHR translocation, a hallmark of alveolar rhabdomyosarcoma (ARMS). Rhabdomyosarcoma (RMS) is the most common paediatric soft-tissue sarcoma, and the third most common extracranial childhood solid tumour. In our experiments, RT-PCR demonstrated the expression of VEGF and VEGFRs by RMS. The addition of exogenous VEGF resulted in ERK-1/2 phosphorylation and cell proliferation, both of which were reduced with VEGFR1 blockade. Treatment with VEGFR1 inhibitor alone slowed RMS growth. All-trans retinoic acid decreased VEGF secretion and slowed growth, which was rescued with exogenous VEGF treatment. These data suggest that autocrine VEGF signalling occurs in RMS and its inhibition may be an effective strategy for rhabdomyosarcoma treatment.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.001
Threshold uncertainty score0.004

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0010.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.034
GPT teacher head0.379
Teacher spread0.346 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations0
Published2004
Admission routes1
Has abstractyes

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