Investigating ICU-acquired Weakness in a 3D Human Skeletal Muscle Microtissue Platform
Bibliographic record
Abstract
Numerous pathologies can lead to progressive muscle weakness and atrophy as secondary consequences. Intensive care unit acquired weakness (ICUAW) is a clinically detected muscle weakness in critically-ill patients, often resulting in persistent disability. We investigated how humoral factors contribute to ICUAW by treating genetically identical 3D-human skeletal muscle microtissues(hMMTs) with sera collected along the treatment of critically-ill patients that may develop ICUAW. Treatment of hMMTs with sera collected within 72 hours of ICU admission with intubation and mechanical ventilation resulted in significantly reduced hMMT myotube diameter, sarcomere integrity and absolute passive force. Comparison of trends in hMMT form and function, when treated with early and later timepoint serum, to clinical outcomes revealed that hMMT passive force outcomes align with clinical outcomes of function measured by Medical Research Council sum score and motor functional independence measure. The next steps will include understanding which molecules are responsible for effects observed in culture.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".