Exploration of riffle scale influences of biotic and abiotic variables on microbial communities of the hyporheic zone
Bibliographic record
Abstract
Terminal restriction fragment length polymorphism (T-RFLP) was optimized for use in the comparison of bacterial communities within various depths and subsurface flow regimes (“zones”) of the hyporheic zone at a riffle scale. The parameters that required optimization included DNA extraction, purification and PCR. The final method developed allowed extraction of sufficient quality and quantity of DNA, and successful amplification of all samples collected. The T-RFLP analysis revealed that microbial communities were heterogeneously distributed throughout the riffle, with more similarity occurring among depths than zones. The physicochemical variables that appeared to have influence on bacteria were primarily depth and sediment particles sizes. No interaction between the composition of bacterial and meiofaunal communities was observed. There was, however, a strong interaction between invertebrates and total microbial density. Hyporheic flows on a riffle were also manipulated to determine the influence of vertical flow patterns on hyporheic communities.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".