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Record W7132894241

The role of Filamin-A in neutrophil chemotaxis

2008· dissertation· W7132894241 on OpenAlexfundno aff
Carol Forster

Bibliographic record

VenueTSpace · 2008
Typedissertation
Language
FieldBiochemistry, Genetics and Molecular Biology
TopicCellular Mechanics and Interactions
Canadian institutionsnot available
FundersCanadian Institutes of Health Research
KeywordsFLNAChemotaxisRHOAFilaminLamellipodiumActinCrosstalkSmall GTPaseNeutrophil extracellular traps
DOInot available

Abstract

fetched live from OpenAlex

Neutrophil chemotaxis is an essential process in maintaining the health of an individual. Although many important mediators involved in neutrophil chemotaxis have been elucidated, no studies have addressed the role that an actin-binding protein, Filamin-A (FLNa) plays in this process. Here we show, using a murine transgenic model in which FLNa is selectively deleted in neutrophils, that FLNa also regulates tail retraction during neutrophil chemotaxis. Neutrophils lacking FLNa show normal polarization and lamellipodium extension, however exhibit obvious defects in tail retraction, found to be a direct result of reduced activation of the small GTPase RhoA and myosin-mediated actin contraction. FLNa appears to be essential for the translocation of p190RhoGAP from the lipid rafts of the cell to allow RhoA activation during neutrophil chemotaxis. Our studies illustrate a novel function for FLNa in neutrophil chemotaxis, which takes us one step closer to fully understanding the mediators involved in the complex process.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.002
Threshold uncertainty score0.005

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.001
Insufficient payload (model declined to judge)0.0010.001

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.006
GPT teacher head0.272
Teacher spread0.266 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations0
Published2008
Admission routes1
Has abstractyes

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