Transcriptional potential of the promyelocytic leukaemia nuclear body
Bibliographic record
Abstract
The promyelocytic leukemia nuclear body (PML NB) is a multi-functional protein based nuclear sub-compartment. The contribution of the PML NB to transcriptional regulation is not clearly defined. Here I have demonstrated that plasmid DNA artificially tethered to PML or the targeting domain of Sp100 became localised to PML NBs in the nuclei of SK-N-SH cells. Targeting SV40 promoter driven reporter constructs to PML NBs resulted in repression of transgene activity whereas CMV promoter driven reporters were upregulated. A minimal eukaryotic promoter was unaffected when targeted to PML NBs. Alteration of the local concentration of PML NB components, including Sp 100 and isoforms of the PML protein, resulted in changes in gene expression of targeted reporters. Moreover, the herpes simplex virus trans-activator, ICP0, increased efficiency of transgene activation of plasmid DNA when tethered to the PML NB. 1 conclude that the PML NB affects gene regulation in a promoter dependent fashion.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.001 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.001 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.001 | 0.000 |
| Research integrity | 0.001 | 0.001 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".