Role of estrogen receptor alpha AF2 domain in aryl hydrocarbon receptor crosstalk
Bibliographic record
Abstract
AHR and ERalpha are ligand-activated transcription factors that mediate the effects of TCDD and estrogens, respectively. My laboratory has previously reported the AHR ligand-dependent recruitment of ERalpha to AHR target genes. To gain better insight into this mechanism of AHR/ERalpha crosstalk, I characterized the ERalpha functional domains involved in this recruitment. I transiently transfected wildtype ERalpha and ERalpha mutants of the AF1, AF2 and DBD domains into HuH-7 cell line and used ChIP analysis followed by Q-PCR to determine recruitment profiles. AF2 mutant displayed significantly lower recruitment to all AHR targets compared to wildtype ERalpha in response to AHR ligand treatments, while the AF1 and DBD mutants displayed less consistent recruitment. Furthermore, pharmacological antagonism of the AF2 inhibited the TCDD-induced ERalpha recruitment supporting the importance of the AF2 in this recruitment. Collectively these results demonstrate the important role of AF2 in the AHR ligand-induced recruitment of ERalpha to AHR targets.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.002 | 0.001 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".