Characterizing the role of Nbp2p in the cell wall integrity pathway of Saccharomyces cerevisiae
Bibliographic record
Abstract
This thesis investigated the role of Nbp2p in the regulation of the Cell Wall Integrity (CWI) MAPK pathway in Saccharomyces cerevisiae. Nbp2p acts as an adaptor protein for the Ptc1p phosphatase, using its SH3 domain to mediate interactions with PxxP-containing target proteins. Co-immunoprecipitation assays provided the first evidence of an interaction between Nbp2p and the MAPK kinase kinase of the CWI pathway, Bck1p. A series of mutations to the Nbp2p SH3 domain and the Bck1p recognition sequence showed a correlation between the affinity of the Nbp2p-Bck1p interaction and the activation of the CWI pathway, and hyperactivation of the pathway was deleterious to cell growth under a variety of cell wall stress conditions. A conserved motif within the Nbp2p N-terminal domain was also identified to be essential for Ptc1p binding. This work demonstrates a novel role for Nbp2p in the CWI pathway and suggests a common targeting mechanism for Ptc1p.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".