Expression allosteric regulation of calcium channel and effect of Ulip-1 on neurogenesis in the adult hippocampus
Bibliographic record
Abstract
This thesis is an investigation of factors involved in the processes of neurodegeneration and neurogenesis in the brain. Three aspects of research were chosen for study in detail. Voltage-dependent calcium channels (VDCCs) are diverse heteromeres that mediate various cellular functions. One major VDCC—the L-type (L-VDCC), is thought to play a role in neurodegeneration and is essential in regulating the influx of calcium into the aging neurons. The expression level of L-VDCCs was investigated in rat aged hippocampus, cortex and cerebellum using specific antibodies, and 3H-isradipine binding assay. The expression level of the α2δ subunit was increased in the aged hippocampus. However, the expression levels of β 3 did not change significantly in the studied regions. The overall density of L-VDCCs did not change. The second topic in this thesis is, the allosteric regulation of calcium channels. Affinity chromatography in conjunction with other techniques such as spectrofluorimetry, radiolabelling, and circular dichroism were used to detect ATP binding and allosteric regulation of the channel. The results indicate that there is no affinity between the fusion protein and Mg-ATP. The third topic in this thesis is the mechanism underlying adult neurogenesis in the hippocampus of Ulip-1 knockout mouse. Ulip-1 protein is highly expressed during neurogenesis in hippocampus. Immunolabelling with Ki67 (a proliferative marker) revealed differences in hippocampal cellular proliferation between −/−, −/+ and +/+ mice. An increased number of Ki67+ cells was found within the proliferative zone (SGZ) of the DG of Ulip-1 knockout mice. Using doublecortin, a marker of immature neurons, it was shown that the number of the cells was markedly increased in −/− mice. Calbindin a marker for mature neurons, showed that intrapyramidal mossy fibers have a defasiculated appearance in CA3 region in −/− mice. These results suggest the involvement of Ulip-1 in cell proliferation and axonal development within the hippocampus.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.001 |
| Insufficient payload (model declined to judge) | 0.002 | 0.001 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".