Trafficking and cell surface stability of the epithelial Na+ channel expressed in epithelial MDCK cells
Bibliographic record
Abstract
The apically located epithelial Na+ channel (αβγENaC) plays a key role in the regulation of salt and fluid transport in the kidney and other epithelia. We generated epithelial Madin-Darby canine kidney (MDCK) cells stably expressing αβγENaC, where each subunit is differentially epitope tagged. ENaC expression was verified by immunoblotting and patch clamp analysis showed the tagged channel is functional. Moreover, we demonstrated apical membrane localization of ENaC in these cells. The glycosylation pattern of the intracellular pool of ENaC revealed sensitivity to endoglycosidase H. The cell surface pool of ENaC was also core glycosylated and lacked detectable endoglycosidase H-resistant channels. Extraction in Triton X-100 demonstrated that both intracellular and cell surface pools of ENaC are largely detergent-soluble. Moreover, flotation assays showed that both intracellular and cell surface pools of this channel are not associated with rafts. The surface pool of ENaC has a short half-life (t1/2 ∼ 1hr) in these cells.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".