The effects of contact guidance and electrical field stimulation on the maturation of cardiac progenitors and cardiomyocytes
Bibliographic record
Abstract
Cardiac tissue engineering requires an appropriate cell source. We investigated the feasibility of various immature cardiac cells, namely neonatal heart cells and embryonic stem cell derived cardiomyocytes and progenitors, in their ability to create the desired adult cardiomyocyte (CM) phenotype. We found that the application of contact guidance and electrical field stimulation improved the alignment and elongation of neonatal cells within a monolayer cultured polyvinyl carbonate or tissue culture polystyrene, as well as the contractile function of the culture. These cells were found to express mature cardiac cell markers such as Troponin I and alpha-sacromeric actinin, in addition to forming gap junctions with neighbouring cells. A similar approach, applied to human embryonic stem cells derived-cardiomyocytes (hESC-CM), shows some promise in maturing these cells into the adult cardiomyocyte phenotype. Finally, we also explored various methods for isolating isl14+ cardiac progenitors from the neonatal rat heart.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".