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Record W7132948540

Computational Tools and Analyses for Improved Inference of Variant Effects

2022· dissertation· W7132948540 on OpenAlexfundno aff
Da Kuang

Bibliographic record

VenueTSpace · 2022
Typedissertation
Language
FieldBiochemistry, Genetics and Molecular Biology
TopicGenomics and Rare Diseases
Canadian institutionsnot available
FundersNational Institutes of HealthCanadian Institutes of Health ResearchVerily Life SciencesAmerican Heart Association
KeywordsExploitInferencePseudogeneMissense mutationComputational modelPipeline (software)Human genomeRobustness (evolution)
DOInot available

Abstract

fetched live from OpenAlex

Personalized medicine requires rapid and accurate classification of pathogenic human variation. With over 50% of clinically interpreted missense variants classified as “variants of uncertain significance” (VUSes), multiple approaches are needed to improve variant interpretation. Multiplexed assays of variant effect (MAVEs) can experimentally test nearly all possible missense variants in selected protein targets, while computational methods seek to infer missense variant impacts using statistical modelling. Here I describe work to assist and exploit both MAVE and computational studies.To assist in planning and to promote collaboration and efficient communication for MAVE studies, I developed: 1) strategies to prioritize genes likely to have a greater impact on clinical variant interpretation, 2) MaveQuest, a resource to help researchers identify MAVE target genes and explore potential assays, and 3) MaveRegistry, a community resource for sharing MAVE progress and finding collaborators. To compare the performance of variant effect predictors and to exploit both experimental and computational information about variant impact, I 1) assessed computational variant effect predictors using a large prospective cohort, and 2) developed a pipeline to screen human pseudogenes for which different genetic variant interpretation might re-classify these pseudogenes to be protein-coding genes, refining human genome annotation.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.012
metaresearch head score (Gemma)0.055
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Theoretical or conceptual · Consensus signal: none
GenreCandidate signal: Methods · Consensus signal: Methods
Teacher disagreement score0.013
Threshold uncertainty score0.062

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0120.055
Meta-epidemiology (narrow)0.0020.002
Meta-epidemiology (broad)0.0020.005
Bibliometrics0.0040.003
Science and technology studies0.0010.002
Scholarly communication0.0040.003
Open science0.0040.003
Research integrity0.0020.005
Insufficient payload (model declined to judge)0.0130.004

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.023
GPT teacher head0.378
Teacher spread0.355 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designTheoretical or conceptual
Domainnot available
GenreMethods

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations0
Published2022
Admission routes1
Has abstractyes

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