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Record W7132992218

Effects of selection procedures on the dynamics of Escherichia coli gene expression

2007· dissertation· W7132992218 on OpenAlex

Why this work is in the frame

A frame that forgets how it found something cannot be audited. These are the routes that admitted this work.

affAt least one author lists a Canadian institution in the pinned OpenAlex snapshot.

Bibliographic record

VenueTSpace · 2007
Typedissertation
Language
FieldBiochemistry, Genetics and Molecular Biology
TopicCancer and biochemical research
Canadian institutionsCanadian HeritageUniversity of Ottawa
FundersFlorida Institute of Technology
KeywordsEscherichia coliGene expressionGeneGreen fluorescent proteinDynamics (music)Synchronization (alternating current)Selection (genetic algorithm)Escherichia coli Proteins
DOInot available

Abstract

fetched live from OpenAlex

The technological advances in the areas of biochemistry and biotechnology, have made it possible to create biological systems for the elucidation of information pertaining to dynamics of gene expression. The bacteria Escherichia coli is often used in such systems, since it is relatively well characterized and simple to work with. A system whereby a plasmid, containing the gene for enhanced green fluorescent protein (EGFP), was inserted into E. coli cells, allowing the expression of the EGFP gene to be monitored by fluorescence microscopy, was created. This system was used to monitor the effects of two selection procedures, (1) cellular synchronization and (2) continuous culture, on the dynamics of gene expression. Cellular synchronization was carried out through the employment of an apparatus called the baby machine and continuous culture was carried out in a device called the chemostat.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

Full frame distilled prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.001
Version: codex-gemma-dda1882f352aValidation status: machine_predicted_unvalidated
Candidate categoriesMeta-epidemiology (narrow)
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.004
Threshold uncertainty score1.000

Codex and Gemma teacher scores by category

CategoryCodexGemma
Metaresearch0.0000.001
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0010.000
Insufficient payload (model declined to judge)0.0000.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.008
GPT teacher head0.341
Teacher spread0.333 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it